Control of in vitro contamination during the establishment of Pyrus communis explants using Plant Preservative MixtureTM

The presence of fungal and bacterial contamination in vitro propagation is a determinant the culture establishment. biocide PPM (Plant Preservative Mixture™) has been utilized to control contamination. According manufacture’s label, it heat-stable, broad-spectrum product, non-selective, that affects directly microorganisms without causing damage plant cells. purpose this study was evaluate influence asepsis on during establishment survival Pyrus communis rootstock explants. Six compositions were tested for explants from two locations (field greenhouse). results showed with Alcohol 70% (1 minute) + NaOCl 2.5% Tween 20 (15 minutes), plus 4.0 ml L-1 added media performed greenhouse plants asepsis, 5% solution bath 2.0 field-grown presented good microbial rate survival. Index terms: Biocide; tissue culture; micropropagation; pear tree; PPM.